Red light cameras at San Jose Blvd and Claire Lane started a 30 day test phase on Monday.
Both northbound and southbound lights at the intersection are now part of the Jacksonville's Red Light Enforcement Program.
The cameras capture and transmit vehicle data to the Jacksonville Sheriff's Office.
The new cameras started a 30 day grace period on Monday. No tickets will be issued, but drivers may see the cameras flash, police said.
The fine for running a red light in Jacksonville is $158.